Apc4 Antibody (CIV1.1)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
CIV1.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Apc4 Antibody (CIV1.1) Summary

Immunogen
C-terminus of APC4
Specificity
CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
ANAPC4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-50105 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Notes

Positive control(s): Purified APC/C by silver and mass spectrometry.

Alternate Names for Apc4 Antibody (CIV1.1)

  • anaphase promoting complex subunit 4
  • anaphase-promoting complex subunit 4
  • APC4Cyclosome subunit 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apc4 Antibody (NBP2-50105)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANAPC4