Apc4 Antibody (CIV1.1) - BSA Free

• Reactivity: Hu
• Applications: WB, IP
• Clone: CIV1.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Apc4 Antibody (CIV1.1) - BSA Free Summary

• Immunogen: C-terminus of Apc4
• Specificity: CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANAPC4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500
• Application Notes: WB - It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently. Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an Apc4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.Positive control(s): Purified APC/C by silver and mass spectrometry.
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Apc4 Antibody (CIV1.1) - BSA Free

• anaphase promoting complex subunit 4
• anaphase-promoting complex subunit 4
• APC4Cyclosome subunit 4

Background

APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. The exact function of APC4 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apc4 Antibody (NBP2-50105)(1)

Publication using NBP2-50105

• De K, Grubb TM, Zalenski AA et al. Hyperphosphorylation of CDH1 in glioblastoma cancer stem cells attenuates APC/CCDH1 activity and pharmacological inhibition of APC/CCDH1/CDC20 compromises viability Mol. Cancer Res. 2019-04-29 [PMID: 31036696] (WB, Human)

Bioinformatics

• Gene Symbol: ANAPC4