Apc11 Antibody - BSA Free

Western Blot: Apc11 Antibody [NBP1-76260] - Analysis of APC11 in HeLa cell lysate with APC11 antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: Apc11 Antibody - BSA Free [NBP1-76260] - Immunofluorescence of Apc11 in 3T3 cells with Apc11 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Apc11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Apc11 Antibody - BSA Free (NBP1-76260) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the center of human APC11. The immunogen is located within amino acids 70 - 120 of APC11. Amino Acid Squence: SLMSREESSRSPDPTPPA
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC11
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Western Blot 1 ug/ml
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Apc11 Peptide (NBP1-76260PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Apc11 Antibody - BSA Free

• anaphase promoting complex subunit 11 (yeast APC11 homolog)
• anaphase promoting complex subunit 11
• anaphase-promoting complex subunit 11
• APC11 anaphase promoting complex subunit 11 homolog
• APC11Hepatocellular carcinoma-associated RING finger protein
• Apc11p
• Cyclosome subunit 11
• HSPC214
• MGC882

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC11 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC11, together with APC2 and Ubc4, form the minimal APC ubiquitin ligase module.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANAPC11
• Uniprot:
  • NP_001002244(Human)