Apc10 Antibody - BSA Free

Western Blot: Apc10 Antibody [NB600-887] - Used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a 26 kDa band corresponding to human APC10 (predicted molecular weight is 21 kDa). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.

Western Blot: Apc10 Antibody [NB600-887] - Antibody was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a- Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa.

Western Blot of Affinity Purified Rabbit Anti-APC10. Lane 1: HeLa whole cell lysate

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Apc10 Antibody - BSA Free Summary

• Description: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Apc10 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human Apc10. (Uniprot: Q9UM13)
• Localization: Centrosomes, mitotic spindles throughout mitosis, kinetochores from prophase to anaphase, midbody in telophase, cytokinesis
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:15000-1:80000
  • Immunohistochemistry 1:250-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:1500
• Application Notes: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide and by western blotting against various cell lysates. Reactivity in other immunoassays is unknown.
• Control:

  Apc10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Apc10 Antibody - BSA Free

• anaphase promoting complex subunit 10
• anaphase-promoting complex subunit 10
• APC10DOC1
• Cyclosome subunit 10
• DKFZP564L0562

Background

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apc10 Antibody (NB600-887)(1)

Publication using NB600-887

• Wang J, Zhang Y, Hou J et al. Ube2s regulates Sox2 stability and mouse ES cell maintenance Cell Death Differ. 2015-08-20 [PMID: 26292759] (WB, Mouse)

Bioinformatics

• Gene Symbol: ANAPC10
• Entrez:
  • 10393(Human)
  • 68999(Mouse)
• Uniprot:
  • Q9UM13(Mouse)