AP2M1 Antibody

Images

 
Western Blot: AP2M1 Antibody [NBP2-15418] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: AP2M1 Antibody [NBP2-15418] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Bovine, Canine, Chicken, Porcine, Rat, Xenopus, MonkeySpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Concentration
1 mg/ml

Order Details

AP2M1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human AP2M1. The exact sequence is proprietary.
Localization
Cell membrane; Membrane; coated pit
Predicted Species
Rat (100%), Porcine (98%), Canine (100%), Chicken (98%), Bovine (100%), Xenopus (98%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AP2M1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (98%), Rhesus Monkey (98%), Chimpanzee (99%).

Alternate Names for AP2M1 Antibody

  • Adaptin-mu2
  • adaptor-related protein complex 2, mu 1 subunit
  • AP-2 mu chain
  • Clathrin assembly protein complex 2 medium chain
  • clathrin coat adaptor protein AP50
  • Clathrin coat assembly protein AP50
  • Clathrin coat-associated protein AP50
  • clathrin-associated/assembly/adaptor protein, medium 1
  • HA2 50 kDa subunit
  • KIAA0109
  • mu subunit
  • Plasma membrane adaptor AP-2 50 kDa protein
  • plasma membrane adaptor AP-2 50kDA protein

Background

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for AP2M1 Antibody (NBP2-15418)

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol AP2M1
Uniprot

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