AP2M1 Antibody (4F1B2) - BSA Free

Western Blot: AP2M1 Antibody (4F1B2) [NBP2-61866] - Analysis using AP2M1 mAb against HEK293 (1) and AP2M1 (AA: 298-435)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: AP2M1 Antibody (4F1B2) [NBP2-61866] - Analysis using AP2M1 mAb against human AP2M1 (AA: 298-435) recombinant protein. (Expected MW is 41.8 kDa)

Western Blot: AP2M1 Antibody (4F1B2) [NBP2-61866] - Analysis using AP2M1 mouse mAb against COS7 (1), SK-Br-3 (2), MCF-7 (3), T47D (4), and HEK293 (5) cell lysate.

ELISA: AP2M1 Antibody (4F1B2) [NBP2-61866] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Pm
• Applications: WB, ELISA
• Clone: 4F1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AP2M1 Antibody (4F1B2) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human AP2M1 (AA: 298-435) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AP2M1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Theoretical MW: 49.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for AP2M1 Antibody (4F1B2) - BSA Free

• Adaptin-mu2
• adaptor-related protein complex 2, mu 1 subunit
• AP-2 mu chain
• Clathrin assembly protein complex 2 medium chain
• clathrin coat adaptor protein AP50
• Clathrin coat assembly protein AP50
• Clathrin coat-associated protein AP50
• clathrin-associated/assembly/adaptor protein, medium 1
• HA2 50 kDa subunit
• KIAA0109
• mu subunit
• Plasma membrane adaptor AP-2 50 kDa protein
• plasma membrane adaptor AP-2 50kDA protein

Background

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP2M1