AP2 alpha Antibody (AP2a 8G8/5)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC
Clone
AP2a 8G8/5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

AP2 alpha Antibody (AP2a 8G8/5) Summary

Immunogen
Bacterialy expressed AP-2a/AP-2g fusion protein.
Localization
Nuclear
Specificity
AP2a 8G8/5 is specific for the alpha form of AP-2.Antigen recovery using microwave or pressure cooking required for paraffin embedded tissue.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TFAP2A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

Positive control(s): Extract from breast cancer cell line ZR75-1.

Alternate Names for AP2 alpha Antibody (AP2a 8G8/5)

  • Activating enhancer-binding protein 2-alpha
  • Activator protein 2
  • AP-2 transcription factor
  • AP2-alpha
  • AP2TFAP-2alpha
  • AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
  • BOFS
  • FLJ51761
  • TFAP2
  • transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
  • transcription factor AP-2-alpha

Background

Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ChIP Video Protocol
ChIP Webinar

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TFAP2A
Entrez