AP2 alpha Antibody (A6/2/2) - BSA Free

Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, CyTOF-ready
• Clone: A6/2/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AP2 alpha Antibody (A6/2/2) - BSA Free Summary

• Immunogen: Peptide to C-Terminal of AP2 alpha coupled to KLH. PNSHTDNNAKSSDKEEKHRK
• Localization: Nuclear
• Specificity: This antibody detects both the alpha and beta forms of AP2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFAP2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Radioimmunoassay 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000
• Application Notes: Recommended for immunohistology using formal saline fixative with calcium on paraffin embedded specimens (10min microwave treatment required). This antibody is CyTOF ready. Positive control(s): Raji cells or breast carcinomas.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AP2 alpha Antibody (A6/2/2) - BSA Free

• Activating enhancer-binding protein 2-alpha
• Activator protein 2
• AP-2 transcription factor
• AP2-alpha
• AP2TFAP-2alpha
• AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
• BOFS
• FLJ51761
• TFAP2
• transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• transcription factor AP-2-alpha

Background

AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFAP2A
• Entrez:
  • 7020(Human)