AP-2 beta/TFAP2B Antibody (AP2b beta 1F3/8)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
AP2b beta 1F3/8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

AP-2 beta/TFAP2B Antibody (AP2b beta 1F3/8) Summary

Immunogen
Truncated AP-2Beta protein prepared from bacteria
Localization
Nuclear
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TFAP2B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
Application Notes
Antigen recovery using microwave or pressure cooking required for paraffin embedded tissue.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Beta 2 Adaptin Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Notes

Positive control(s): Human breast cancer line MDA MB 453.

Alternate Names for AP-2 beta/TFAP2B Antibody (AP2b beta 1F3/8)

  • activating enhancer binding protein 2 beta
  • Activating enhancer-binding protein 2-beta
  • AP2 beta
  • AP-2 beta
  • AP-2B
  • AP2-B
  • AP2-beta
  • CHAR
  • MGC21381
  • TFAP2B
  • transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
  • transcription factor AP-2 beta (activating enhancer-binding protein 2 beta)
  • transcription factor AP-2-beta

Background

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for AP-2 beta/TFAP2B Antibody (NB100-2640) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2640AF350
Alexa Fluor 405 NB100-2640AF405
Alexa Fluor 488 NB100-2640AF488
Alexa Fluor 532 NB100-2640AF532
Alexa Fluor 594 NB100-2640AF594
Alexa Fluor 647 NB100-2640AF647
Alexa Fluor 700 NB100-2640AF700
Alexa Fluor 750 NB100-2640AF750
Biotin NB100-2640B
DyLight 350 NB100-2640UV
DyLight 405 NB100-2640V
DyLight 488 NB100-2640G
DyLight 550 NB100-2640R
DyLight 594 NB100-2640DL594
DyLight 650 NB100-2640C
DyLight 680 NB100-2640FR
DyLight 755 NB100-2640IR
FITC NB100-2640F
HRP NB100-2640H
Janelia Fluor 549 NB100-2640JF549
Janelia Fluor 646 NB100-2640JF646

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Bioinformatics

Gene Symbol TFAP2B
Entrez
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