ANP32A Knockout 293T Cell Lysate

Product Discontinued

ANP32A Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,8 bp deletion in exon 1
• Gene: ANP32A

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for ANP32A Knockout 293T Cell Lysate

• acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• acidic leucine-rich nuclear phosphoprotein 32 family member A
• Acidic nuclear phosphoprotein pp32
• cerebellar leucine rich acidic nuclear protein
• I1PP2A
• inhibitor-1 of protein phosphatase-2A
• LANPPutative HLA-DR-associated protein I
• Leucine-rich acidic nuclear protein
• MAPMC15orf1
• mapmodulin
• PHAP1MGC150373
• PHAPIMGC119787
• Potent heat-stable protein phosphatase 2A inhibitor I1PP2A
• PP32
• putative human HLA class II associated protein I

Background

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ANP32A