Annexin A2 Antibody

Western Blot: Annexin A2 Antibody [NB100-881] - Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Bv, Rt, Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Annexin A2 Antibody Summary

• Immunogen: Peptide with sequence STVHEILCKLSLEGD corresponding to N-Terminus according to NP_004030.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001002858.1; NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein.
• Predicted Species: Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANXA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.5 - 3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in A549, HeLa and NIH3T3 lysates (calculated MW of 38.6 kDa band according to NP_004030.1 and NP_001002857.1). IHC-P usage is reported in scientific literature (PMID: 25827591).
• Control:

  Annexin A2 Overexpression Lysate

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25827591).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Annexin A2 Antibody

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A2 Antibody (NB100-881)(2)

Publications using NB100-881

• Senthilkumaran C, Hewson J, Ollivett TL, Bienzle D. Localization of annexins A1 and A2 in the respiratory tract of healthy calves and those experimentally infected with Mannheimia haemolytica Veterinary Research. 2015-02-01 [PMID: 25827591] (IHC-P, Bovine)
  
  Details:
  Annexin A2 antibody used for IHC-P on tracheal, bronchial and lung tissues from 2-month-old healthy male Holstein calves which were experimentally infected or not with Mannheimia haemolytica. The IHC-P assay involved heat-induced epitope retrieval with pressure cooker/approximately 120C temperature using sodium citrate buffer pH 6, treatment of slides with Dako's endogenous enzyme blocker and serum-free protein blocker, incubation of slides with primary antibody at 1 ug/150 ul concentration for ON at 4C, detection with HRP conjugated secondary antibody and NovaRED chromogen followed by hematoxylin counterstain.
• Takahashi S, Reddy SV, Chirgwin JM et al. Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption. J Biol Chem 1994-11-18 [PMID: 7961821]

Bioinformatics

• Gene Symbol: ANXA2
• Entrez:
  • 302(Human)
  • 12306(Mouse)
  • 56611(Rat)
• Uniprot:
  • P07355(Human)