Annexin A11 Antibody (OTI1C6)

Western Blot: Annexin A11 Antibody (1C6) [NBP2-02342] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Annexin A11.

Immunocytochemistry/Immunofluorescence: Annexin A11 Antibody (1C6) [NBP2-02342] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Annexin A11.

Immunohistochemistry-Paraffin: Annexin A11 Antibody (1C6) [NBP2-02342] - Human prostate tissue.

Flow Cytometry: Annexin A11 Antibody (1C6) [NBP2-02342] - HEK293T cells transfected with either pCMV6-ENTRY Annexin A11.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Annexin A11 mouse monoclonal, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: OTI1C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Annexin A11 Antibody (OTI1C6) Summary

• Immunogen: Full length human recombinant protein of human ANXA11 (NP_001148) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-200
  • Immunohistochemistry-Paraffin 1:100-200
  • Western Blot 1:2000
• Theoretical MW: 54.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Annexin A11 Antibody (OTI1C6)

• Annexin A11
• Annexin-11
• ANX1156-kD
• ANXA11
• CAP50
• CAP-50

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA11
• Entrez:
  • 994(Human)