Annexin A10 Antibody (SC06-02)

Western Blot: Annexin A10 Antibody (SC06-02) [NBP2-67600] - Western blot analysis of Annexin A10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat An

Immunohistochemistry-Paraffin: Annexin A10 Antibody (SC06-02) [NBP2-67600] - Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Annexin A10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-57, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Annexin A10 Antibody (SC06-02) [NBP2-67600] - Analysis of Annexin A10 on different lysates using anti-Annexin A10 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat

Immunohistochemistry-Paraffin: Annexin A10 Antibody (SC06-02) [NBP2-67600] - Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Annexin A10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-57, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Annexin A10 Antibody (SC06-02) [NBP2-67600] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Annexin A10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-57, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Annexin A10 Antibody (SC06-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Annexin A10 aa 30-220 / 324. (SwissProt: Q9UJ72 Human; SwissProt: Q9QZ10 Mouse; Unigene:101584 Rat)
• Localization: Cytoplasm, nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANXA10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Annexin A10 Antibody (SC06-02)

• annexin 14
• Annexin A10
• annexin-10
• annexin-14
• ANX14
• ANX14Annexin-10
• ANXA10

Background

Annexin A10 encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The function of this gene has not yet been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA10