Annexin A1 Antibody

Western Blot: Annexin A1 Antibody [NBP1-18842] - Analysis of Pig Spleen lysate (35ug protein in RIPA buffer).

Western Blot: Annexin A1 Antibody [NBP1-18842] - Analysis of Rat Spleen lysate (35ug protein in RIPA buffer).

Western Blot: Annexin A1 Antibody [NBP1-18842] - Analysis of K562 (A) and NIH3T3 (B) lysates (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Annexin A1 Antibody Summary

• Description: Novus Biologicals Goat Annexin A1 Antibody (NBP1-18842) is a polyclonal antibody validated for use in WB and ELISA. Anti-Annexin A1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CQAILDETKGDYEK corresponding to C-Terminus according to NP_000691.1.
• Epitope: CQAILDETKGDYEK
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANXA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in lysates of K562 and NIH3T3 (calculated MW of 38.7 kDa band according to human NP_000691.1 and mouse NP_034860.2). approx. 37 kDa band observed in rat and porcine spleen lysates (calculated MW of 38.7 kDa band according to porcine NP_001157470.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Annexin A1 Antibody

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A1 Antibody (NBP1-18842)(3)

Publications using NBP1-18842

• de Oliveira B M, Matsumura C Y et al. Quantitative proteomic analysis reveals metabolic alterations, calcium dysregulation, and increased expression of extracellular matrix proteins in laminin alpha 2 chain-deficient muscle. Mol Cell Proteomics 2014-01-11 [PMID: 24994560] (WB, Mouse)
• Fan X, Krahling S, Smith D et al. Macrophage surface expression of annexins I and II in the phagocytosis of apoptotic lymphocytes. Mol Biol Cell 2004-06-01 [PMID: 15064349]
• Paweletz CP, Ornstein DK, Roth MJ et al. Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma. Cancer Res 2000-11-01 [PMID: 11103786]

Bioinformatics

• Gene Symbol: ANXA1
• Entrez:
  • 301(Human)
  • 16952(Mouse)
  • 25380(Rat)
• Uniprot:
  • NP_000691.1(Human)