![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0002.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Ankyrin 3 Antibody - BSA Free (NBP3-05550) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANK3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 5mM Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ANK3 |
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Immunofluorescent analysis of a rat brain section stained with Ankyrin 3 Antibody at a dilution of 1:1,000, in red and costained with mouse mAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 antibody stains axonal initial segments, while the MAP2 antibody labels MAP2 protein expressed in the perikarya and dendrites of most neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Immunofluorescent analysis of a rat brain section stained with Ankyrin 3 Antibody at a dilution of 1:1,000, in red and costained with mouse mAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 antibody stains axonal initial segments, while the MAP2 antibody labels MAP2 protein expressed in the perikarya and dendrites of most neurons.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
