![Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05528-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 2A8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse Ankyrin 3 Antibody (2A8) - BSA Free (NBP3-05528) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ANK3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 5mM Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ANK3 |
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")