Recombinant Human ANKRD54 His Protein

SDS-Page: ANKRD54 Protein [NBP2-22688]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human ANKRD54 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-300 of Human ANKRD54
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAAAGD ADDEPRSGHS SSEGECAVAP EPLTDAEGLF SFADFGSALG GGGAGLSGRA SGGAQSPLRY LHVLWQQDAE PRDELRCKIP AGRLRRAARP HRRLGPTGKE VHALKRLRDS ANANDVETVQ QLLEDGADPC AADDKGRTAL HFASCNGNDQ IVQLLLDHGA DPNQRDGLGN TPLHLAACTN HVPVITTLLR GGARVDALDR AGRTPLHLAK SKLNILQEGH AQCLEAVRLE VKQIIHMLRE YLERLGQHEQ RERLDDLCTR LQMTSTKEQV DEVTDLLASF TSLSLQMQSM EKR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ANKRD54
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 34.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human ANKRD54 His Protein

• ankyrin repeat domain 54
• LIARankyrin repeat domain-containing protein 54

Background

ANKRD54, also known as LIAR, contains 4 ANK repeats. This protein plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. This protein interacts (via ankyrin repeat region) with LYN (via SH3-domain) in an activation-independent status of LYN. It forms a multiprotein complex with LYN and HCLS1. ANKRD54 also interacts with TSN2, VAV1, DBNL AND LASP1. Recombinant human ANKRD54 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ANKRD54