ANKRD15 Antibody - BSA Free

Western Blot: ANKRD15 Antibody [NBP1-03345] - Detection of Human KANK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KANK1 antibody NBP1-03345 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: ANKRD15 Antibody [NBP1-03345] - Detection of Human KANK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- KANK1 antibody NBP1-03345 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KANK1 was also immunoprecipitated by another rabbit anti-KANK1 antibody which recognizes an upstream epitope.

ANKRD15 Antibody [NBP1-03345] - Detection of human KANK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KANK1 antibody NBP1-03345 (lot NBP1-03345-2) used for IP at 6 ug per reaction. KANK1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-03345-1) and another rabbit anti-KANK1 antibody. For blotting immunoprecipitated KANK1, NBP1-03345 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ANKRD15 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ANKRD15 Antibody - BSA Free (NBP1-03345) is a polyclonal antibody validated for use in WB and IP. Anti-ANKRD15 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1302 and 1352 of human KN motif and ankyrin repeat domains 1 using the numbering given in entry NP_055973.2 (GeneID 23189).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KANK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ANKRD15 Antibody - BSA Free

• ANKRD15ankyrin repeat domain 15
• Ankyrin repeat domain-containing protein 15
• KANKDKFZp451G231
• KIAA0172MGC43128
• Kidney ankyrin repeat-containing protein
• KN motif and ankyrin repeat domain-containing protein 1
• KN motif and ankyrin repeat domains 1

Background

The ANKRD15 gene encodes a protein containing four ankyrin repeat domains in its C-terminus. The suggested role for this protein is in tumorigenesis of renal cell carcinoma. Two alternatively spliced transcript variants encoding different isoforms have been iden

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ANKRD15 Antibody (NBP1-03345)(1)

Publication using NBP1-03345

• Li CC, Kuo JC, Waterman CM et al. Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing. Proc Natl Acad Sci U S A 2011-11-01 [PMID: 22084092]

Bioinformatics

• Gene Symbol: KANK1
• Entrez:
  • 23189(Human)
• Uniprot:
  • Q14678(Human)