Amphiphysin/AMPH Antibody (3)

Product Discontinued

Amphiphysin/AMPH Antibody (3) Summary

• Immunogen: Recombinant human Amphiphysin.
• Specificity: Detects a ~128 kD protein, corresponding to the apparent molecular mass of Amphiphysin I on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, hamster, monkey and rabbit origins. A 108 kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of Amphiphysin I (residues 545-695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: AMPH
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.5 ug/ml

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1mM PMSF and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Amphiphysin/AMPH Antibody (3)

• AMPH
• AMPH1
• amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
• amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
• amphiphysin I
• Amphiphysin

Background

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMPH
• Entrez:
  • 273(Human)
• Uniprot:
  • P49418(Human)