Amphiphysin/AMPH Antibody (3)

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Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody (3) [NBP1-52349] - Analysis of anti-AMPH / Amphiphysin antibody with human brain, cerebellum at concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody (3) [NBP1-52349] - Staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ha, Pm, RbSpecies Glossary
Applications WB, IHC-P, IP
Clone
3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Amphiphysin/AMPH Antibody (3) Summary

Immunogen
Recombinant human Amphiphysin.
Specificity
Detects a ~128 kD protein, corresponding to the apparent molecular mass of Amphiphysin I on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, hamster, monkey and rabbit origins. A 108 kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of Amphiphysin I (residues 545-695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
AMPH
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1mM PMSF and 50% Glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Amphiphysin/AMPH Antibody (3)

  • AMPH
  • AMPH1
  • amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
  • amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
  • amphiphysin I
  • Amphiphysin

Background

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AMPH
Entrez
Uniprot