Aminoacylase/ACY1 Antibody

Western Blot: Aminoacylase/ACY1 Antibody [H00000095-D01P] - Analysis of ACY1 expression in mouse liver.

Western Blot: Aminoacylase/ACY1 Antibody [H00000095-D01P] - Analysis of ACY1 expression in MCF-7.

Western Blot: Aminoacylase/ACY1 Antibody [H00000095-D01P] - Analysis of ACY1 expression in NIH/3T3.

Western Blot: Aminoacylase/ACY1 Antibody [H00000095-D01P] - Analysis of ACY1 expression in human liver.

Western Blot: Aminoacylase/ACY1 Antibody [H00000095-D01P] - Analysis of ACY1 expression in transfected 293T cell line by ACY1 polyclonal antibody.Lane 1: ACY1 transfected lysate(45.90 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Aminoacylase/ACY1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ACY1 (NP_000657.1, 1 a.a. - 408 a.a.) full-length human protein. MTSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS
• Specificity: ACY1 - aminoacylase 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACY1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Aminoacylase/ACY1 Antibody

• ACY1
• ACY-1
• ACY1D
• acylase
• aminoacylase 1
• Aminoacylase
• aminoacylase-1
• EC 3.5.1
• EC 3.5.1.14
• N-acyl-L-amino-acid amidohydrolase

Background

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACY1
• Entrez:
  • 95(Human)
• Uniprot:
  • NP_000657.1(Human)