AMIGO2 Antibody - BSA Free

Western Blot: AMIGO2 Antibody [NB300-247] - WB detection of AMIGO2 / Alivin in 50 ug of murine brain lysate using antibody NB300-247 at a dilution of 1:500. This antibody detect bands at 63 kDa and 66 kDa positions in mouse brain tissue.

Product Discontinued

AMIGO2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide mapping to residues 449-464 [TGDASADDRKAGKRVV] of mouse and rat Alivin 1.
• Specificity: Reacts with residues 449-464 [TGDASADDRKAGKRVV] of mouse and rat Alivin 1. Detects bands of 63 ands 66 kDa in brain with the majority of the immunoreactivity in the nuclear fraction.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMIGO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Western blot - Use at 1:500 dilution. This antibody has only been tested in Western Blot. The investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse and rat. Other species have not been tested. There is 81% sequence identity to human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMIGO2 Antibody - BSA Free

• adhesion molecule with Ig-like domain 2
• ALI1amphoterin induced gene 2
• alivin 1
• Alivin-1
• AMIGO2
• AMIGO-2
• DEGAamphoterin-induced protein 2
• differentially expressed in gastric adenocarcinoma
• Differentially expressed in gastric adenocarcinomas
• transmembrane protein AMIGO2

Background

A novel primary response gene, Alivin 1 (ali1), is an activity-dependent gene and promotes survival of neurons. Sequence analyses reveal that rat, mouse, and human Ali1 proteins contain seven leucine-rich repeats, one IgC2-like loop and a transmembrane domain, and display homology to Kek and Trk families. Results suggest that expression of ali1 promotes depolarization-dependent survival of the granule neuron. Mouse ali1 was mapped to a locus approximately 55.3 cM from the centromere on chromosome 15 that is syntenic to positional candidate loci for familial Alzheimer's disease type 5 and Parkinson's disease 8 on human chromosome 12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMIGO2
• Entrez:
  • 105827(Mouse)
  • 300186(Rat)
• Uniprot:
  • Q80ZD9()
  • Q7TNJ4(Rat)