ALS2CR8 Antibody (2A3) - Azide and BSA Free

Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - ALS2CR8 monoclonal antibody (M01), clone 2A3 Analysis of ALS2CR8 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of monoclonal antibody to ALS2CR8 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of monoclonal antibody to ALS2CR8 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.

Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of ALS2CR8 over-expressed 293 cell line, cotransfected with ALS2CR8 Validated Chimera RNAi ( Cat # H00079800-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALS2CR8 monoclonal antibody (M01), clone 2A3 (Cat # H00079800-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of ALS2CR8 expression in transfected 293T cell line by ALS2CR8 monoclonal antibody (M01), clone 2A3.Lane 1: ALS2CR8 transfected lysate(79.8 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: ALS2CR8 Antibody (2A3) [H00079800-M01] - Detection limit for recombinant GST tagged ALS2CR8 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 2A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ALS2CR8 Antibody (2A3) - Azide and BSA Free Summary

• Immunogen: ALS2CR8 (NP_079020, 621 a.a. ~ 718 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CLTQNNSTASTMGNLPEPDQNLVAMDELVEVGDVEDTGNLEGTVHRILLGDVQTIPIQIIDNHSALIEENPESTISVSQVKQEPKEPALSMEAKNCGL
• Specificity: ALS2CR8 - amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CARF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation, immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ALS2CR8 Antibody (2A3) - Azide and BSA Free

• amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8
• amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein
• Calcium-response factor
• CaRF
• CARFDKFZp667N246
• FLJ12675
• FLJ21579
• NYD-SP24
• Testis development protein NYD-SP24

Background

ALS2CR8 may be a transcription factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARF
• Entrez:
  • 79800(Human)
• OMIM:
  • 607586(Human)
• Uniprot:
  • NP_079020(Human)