ALPK1 Antibody

Western Blot: ALPK1 Antibody [NBP1-83594] - Analysis in control (vector only transfected HEK293T lysate) and ALPK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: ALPK1 Antibody [NBP1-83594] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ALPK1 Antibody [NBP1-83594] - Staining of human kidney shows moderate positivity in cells in tubules and glomeruli.

Product Discontinued

ALPK1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SYSSSNDCPPELKNLHLCEAKEAFEIGLLTKRDDEPVTGKQELHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALPK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 27829216). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23276153)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ALPK1 Antibody

• alpha-kinase 1
• alpha-protein kinase 1
• ALPK1
• Chromosome 4 kinase
• EC 2.7.11.-
• FLJ22670
• KIAA1527
• KIAA15278430410J10Rik
• LAK
• LAKFLJ22670
• lymphocyte alpha-kinase
• Lymphocyte alpha-protein kinase
• ROSAH

Background

Unlike most eukaryotic kinases, alpha kinases, such as LAK, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALPK1 Antibody (NBP1-83594)(2)

Publications using NBP1-83594

• Strietz J, Stepputtis SS, Preca BT et al. ERN1 and ALPK1 inhibit differentiation of bi-potential tumor-initiating cells in human breast cancer. Oncotarget. 2016-11-04 [PMID: 27829216] (WB, Human)
• Fagerberg L, Oksvold P, Skogs M et al. Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP). J Proteome Res 2013-06-07 [PMID: 23276153]

Bioinformatics

• Gene Symbol: ALPK1