AlphaB Crystallin/CRYAB Antibody (731502)

Western blot shows lysates of rat heart tissue, mouse heart tissue, and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human AlphaB Crystallin/CRYAB and recombinant human AlphaA Crystallin/CRYAA (5 ng/lane) were included. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 28 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 731502
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AlphaB Crystallin/CRYAB Antibody (731502) Summary

• Immunogen: E. coli-derived recombinant human Crystallin/CRYAB
  Met1-Lys175
  Accession # P02511
• Specificity: Detects human AlphaB Crystallin/CRYAB in direct ELISAs and human, mouse, and rat AlphaB Crystallin/CRYAB in Western blots. In direct ELISAs, no cross-reactivity with recombinant human AlphaA Crystallin/CRYAA is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRYAB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AlphaB Crystallin/CRYAB Antibody (731502)

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

CRY-alpha B (crystalline alpha -B chain/CRYAB; also HspB5) is a 22-23 kDa member of the HSP20 family of proteins.  It has widespread expression, and is found in lens epithelium where it noncovalently oligomerizes with CRYAA to generate a transparent 350-1000 kDa alpha -crystalline protein complex.  Human CRYAB is 175 amino acids (aa) in length. It contains an alpha -crystalline Hsp domain over aa 66-149.  Multiple post-translational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved.  It may also be phosphorylated at Ser45 and 59, be potentially O-GlcNAc modified at Thr158, 162 or 170, and acetylated at Lys92.  An alternate start site at Met68 may be accompanied by a 47 aa substitution for aa 109-175.  Full-length human CRYAB shares 54% aa sequence identity with CRYAA, and 98% aa identity with mouse CRYAB.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (MAB4849)(3)

Publications using MAB4849

• E Adriaensse, B Asselbergh, P Rivera-Mej, S Bervoets, L Vendredy, V De Winter, K Spaas, R de Rycke, G van Isterd, F Impens, T Langer, V Timmerman Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space Nature Cell Biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36690850] (Western Blot, Mouse)
• T Reid Reid Alderson, Elias Adriaenssens, Bob Asselbergh, Iva Pritišanac, Jonas Van Lent, Heidi Y Gastall et al. A weakened interface in the P182L variant of HSP27 associated with severe Charcot‐Marie‐Tooth neuropathy causes aberrant binding to interacting proteins The EMBO Journal 2021-04-15 [PMID: 33644875]
• A Rouhiainen, N Kulesskaya, M Mennesson, Z Misiewicz, T Sipilä, E Sokolowska, K Trontti, L Urpa, W McEntegart, S Saarnio, P Hyytiä, I Hovatta The bradykinin system in stress and anxiety in humans and mice Sci Rep, 2019-12-19;9(1):19437. 2019-12-19 [PMID: 31857655] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CRYAB
• Uniprot:
  • P02511(Human)