Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein

Images

 
In vitro assay: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein [NBP3-14766] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein ...read more
SDS-Page: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein [NBP3-14766] - SDS-PAGE of ~14 kDa A53T alpha-Synuclein Monomer (NBP3-14766)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, In vitro, In vivo, PAGE

Order Details

Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein Summary

Description
A full length recombinant protein of Human alpha-Synuclein

Source: E. coli

Uniprot ID: P37840 

Amino Acid Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Localization
Cytoplasm, Membrane, Nucleus
Preparation
Method
Ion-exchange Purified
Details of Functionality
100 uM A53T alpha-Synuclein protein monomer seeded with 10 uM A53T alpha-Synuclein protein pre-formed fibrils in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated a fluorescence intensity of 28 000 RFU after incubation at 37C with shaking at 600 rpm for 56 hours. Fluorescence measured by excitation at 450 nm and emission at 485 nm on a microplate reader.
Protein/Peptide Type
Recombinant Protein
Gene
SNCA
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • In vitro assay
  • In vivo assay
  • SDS-Page
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4
Preservative
No Preservative
Purity
>95%, by SDS-PAGE

Notes

Please note that the 200ug and 500ug sizes are sent in 2x100ug and 5x100ug vials, respectively

Alternate Names for Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein

  • alpha-Synuclein
  • I+/--synuclein
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • PARK1
  • PARK4
  • PD1
  • SNCA
  • synuclein alpha-140
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha
  • truncated alpha synuclein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-82245
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, KD, Simple Western, WB
NB300-268
Species: Bv, Ce, Hu, I, Mu, Pl
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
NBP3-05529
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, WB
NB300-270
Species: Ch, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, In vitro, Simple Western, WB
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-55955
Species: Hu
Applications: ICC/IF, IHC, IHC-P
BC100-494
Species: Dr, Hu, Mu, Rb, Rt
Applications: EM, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PEP-ELISA, PAGE, WB
NB600-1160
Species: Bv, Ca, Hu, Mu, Po, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-66861
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP3-13106
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56148
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1438
Species: Hu
Applications: IHC, WB
NBP2-67569
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-74510
Species: Hu, Mu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP2-27544
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP2-15895
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-534
Species: Hu, Mu, Po, V-Vi
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA, WB

Publications for alpha-Synuclein Recombinant Protein (NBP3-14766) (0)

There are no publications for alpha-Synuclein Recombinant Protein (NBP3-14766).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for alpha-Synuclein Recombinant Protein (NBP3-14766) (0)

There are no reviews for alpha-Synuclein Recombinant Protein (NBP3-14766). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for alpha-Synuclein Recombinant Protein (NBP3-14766). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Additional alpha-Synuclein Products

Bioinformatics Tool for alpha-Synuclein Recombinant Protein (NBP3-14766)

Discover related pathways, diseases and genes to alpha-Synuclein Recombinant Protein (NBP3-14766). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha-Synuclein Recombinant Protein (NBP3-14766)

Discover more about diseases related to alpha-Synuclein Recombinant Protein (NBP3-14766).
 

Pathways for alpha-Synuclein Recombinant Protein (NBP3-14766)

View related products by pathway.

PTMs for alpha-Synuclein Recombinant Protein (NBP3-14766)

Learn more about PTMs related to alpha-Synuclein Recombinant Protein (NBP3-14766).
 

Research Areas for alpha-Synuclein Recombinant Protein (NBP3-14766)

Find related products by research area.

Blogs on alpha-Synuclein.

Mechanisms of neurodegeneration: Mitochondrial dysfunction and oxidative stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol SNCA