Synuclein alpha/beta Antibody (3B6) - BSA Free

Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western Blot: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Synuclein alpha/beta Antibody (3B6) - BSA Free Summary

• Description: Novus Biologicals Mouse Synuclein alpha/beta Antibody (3B6) - BSA Free (NBP1-22957) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This Synuclein alpha/beta Antibody (3B6) was developed against recombinant human Synuclein alpha/beta (119-140aa) purified from E. coli.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNCA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 2-5ug
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Synuclein alpha/beta Antibody (3B6) - BSA Free

• beta-synuclein
• I(2)-synuclein
• I+/--synuclein
• NACP
• non A-beta component of AD amyloid
• PARK1
• PD1
• synuclein alpha-140
• synuclein, alpha (non A4 component of amyloid precursor)
• truncated alpha synuclein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNCA
• Entrez:
  • 6622(Human)
• Uniprot:
  • NP_000336(Human)