Alpha Fodrin Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Alpha Fodrin Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues around the calpain cleavage site, tyrosine 1176 in human Alpha Fodrin. This sequence has high homology with similar regions in rat and mouse Alpha Fodrin, but is not found in other spectrin family members. [UniProt# Q13813]
• Specificity: The antibody detects a 136 kDa cleaved fragment corresponding to Alpha Fodrin on SDS-PAGE immunoblots of mouse diaphram treated with thapsigargin. The antibody only detects cleaved Alpha Fodrin and does not detect full length Alpha Fodrin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTAN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 136 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Alpha Fodrin Antibody

• Alpha-II spectrin
• EIEE5
• FLJ17738
• FLJ44613
• Fodrin alpha chain
• NEAS
• spectrin alpha chain, brain
• spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Spectrin, non-erythroid alpha chain
• SPTA2

Background

SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTAN1