Alpha Fodrin Antibody (3D7) [DyLight 405]

Images

 
There are currently no images for Alpha Fodrin Antibody (NBP1-92689V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ICC/IF
Clone
3D7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Alpha Fodrin Antibody (3D7) [DyLight 405] Summary

Immunogen
Recombinant construct (expressed in and purified from E. coli) containing the 13th, 14th and 15th spectrin repeats of hiuman human alpha Fodrin (amino acids 1398-1685) [UniProt# Q13813].
Localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Note: Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPTAN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Alpha Fodrin (3D7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 240 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alpha Fodrin Antibody (3D7) [DyLight 405]

  • Alpha-II spectrin
  • EIEE5
  • FLJ17738
  • FLJ44613
  • Fodrin alpha chain
  • NEAS
  • spectrin alpha chain, brain
  • spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
  • Spectrin, non-erythroid alpha chain
  • SPTA2

Background

Alpha Fodrin (SPTAN1; also called non-erythroid alpha spectrin) belongs to spectrin family of proteins which are widely distributed filamentous proteins implicated in generating a supporting/organizing scaffold for cell membranes, and SPTAN1 protein is involved in processes such as secretion, interactions with calmodulin in Ca2+ dependent manner and Ca2+ dependent cytoskeletal movements at the membranes. SPTAN1 interacts with several proteins including ACP1, CALM, EMD, TRPC4 etc. and forms a component of a complex that contains several other proteins such as ACTN4, CASK, IQGAP1, MAGI2, NPHS1 and SPTBN1. SPTAN1 cleavage accompanies apoptosis, induced by activation via CD3/TCR complex, Fas/CD95 ligation and other Fas-expressing cells or through cellular exposure to staurosporine, dexamethasone, synthetic ceramide etc. Major products of SPTAN1 cleavage are N-terminal/C-terminal fragments, and phosphorylation of Tyr-1176 has been shown to decrease its sensitivity to cleavage by calpain in vitro. Defects in SPTAN1 have been linked to EIEE5 (epileptic encephalopathy early infantile type 5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NB120-3515
Species: Hu, Rt, Po, Bv, Ca, Fi, Rb
Applications: WB, IHC, IHC-Fr, IHC-P, ICC
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-48002
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IHC-P, IP, PA
NBP1-33548
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-86088
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-43728
Species: Hu
Applications: WB, ICC/IF
NBP2-14871
Species: Hu, Mu
Applications: WB, ICC/IF
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC, IF
NBP1-98474
Species: Hu
Applications: WB
NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56118
Species: Hu, Mu, Rt, Ca, Ge
Applications: IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Alpha Fodrin Antibody (NBP1-92689V) (0)

There are no publications for Alpha Fodrin Antibody (NBP1-92689V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Alpha Fodrin Antibody (NBP1-92689V) (0)

There are no reviews for Alpha Fodrin Antibody (NBP1-92689V). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Alpha Fodrin Antibody (NBP1-92689V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP1-92689UV
DyLight 488 Labeled NBP1-92689G
DyLight 680 Labeled NBP1-92689FR
DyLight 755 Labeled NBP1-92689IR
FITC Labeled NBP1-92689F

Additional Alpha Fodrin Products

Bioinformatics Tool for Alpha Fodrin Antibody (NBP1-92689V)

Discover related pathways, diseases and genes to Alpha Fodrin Antibody (NBP1-92689V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Alpha Fodrin Antibody (NBP1-92689V)

Discover more about diseases related to Alpha Fodrin Antibody (NBP1-92689V).
 

Pathways for Alpha Fodrin Antibody (NBP1-92689V)

View related products by pathway.

Research Areas for Alpha Fodrin Antibody (NBP1-92689V)

Find related products by research area.

Blogs on Alpha Fodrin

There are no specific blogs for Alpha Fodrin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Alpha Fodrin RNAi
H00006709-R01-20nmol

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Alpha Fodrin Antibody (3D7) [DyLight 405] and receive a gift card or discount.

Bioinformatics

Gene Symbol SPTAN1