Alpha Fodrin Antibody (3D7) [mFluor Violet 610 SE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 3D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

Alpha Fodrin Antibody (3D7) [mFluor Violet 610 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant construct (expressed in and purified from E. coli) containing the 19th and 20th spectrin repeats and C-terminal EF hand domains of human alpha Fodrin (amino acids 2086-2447) [UniProt# Q13813].
• Localization: Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Note: Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPTAN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-92689. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alpha Fodrin Antibody (3D7) [mFluor Violet 610 SE]

• Alpha-II spectrin
• EIEE5
• FLJ17738
• FLJ44613
• Fodrin alpha chain
• NEAS
• spectrin alpha chain, brain
• spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Spectrin, non-erythroid alpha chain
• SPTA2

Background

SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTAN1