Alpha Fodrin Antibody (3D7) [HRP]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 3D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

Alpha Fodrin Antibody (3D7) [HRP] Summary

• Immunogen: Recombinant construct (expressed in and purified from E. coli) containing the 19th and 20th spectrin repeats and C-terminal EF hand domains of human alpha Fodrin (amino acids 2086-2447) [UniProt# Q13813].
• Localization: Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Note: Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPTAN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for Alpha Fodrin Antibody (3D7) [HRP]

• Alpha-II spectrin
• EIEE5
• FLJ17738
• FLJ44613
• Fodrin alpha chain
• NEAS
• spectrin alpha chain, brain
• spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Spectrin, non-erythroid alpha chain
• SPTA2

Background

SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTAN1