Alpha Fodrin Antibody (3D7) [DyLight 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 3D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Alpha Fodrin Antibody (3D7) [DyLight 488] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Recombinant construct (expressed in and purified from E. coli) containing the 19th and 20th spectrin repeats and C-terminal EF hand domains of human alpha Fodrin (amino acids 2086-2447) [UniProt# Q13813].
• Localization: Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Note: Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPTAN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This Alpha Fodrin (3D7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 240 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Alpha Fodrin Antibody (3D7) [DyLight 488]

• Alpha-II spectrin
• EIEE5
• FLJ17738
• FLJ44613
• Fodrin alpha chain
• NEAS
• spectrin alpha chain, brain
• spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Spectrin, non-erythroid alpha chain
• SPTA2

Background

SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTAN1