Alpha Dystroglycan Antibody

Images

 
Western Blot: Alpha Dystroglycan Antibody [NBP2-14868] - U87-MG whole cell extracts and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Alpha Dystroglycan Antibody [NBP2-14868] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Dystroglycan protein stained by alpha Dystroglycan antibody ...read more
Immunohistochemistry-Paraffin: Alpha Dystroglycan Antibody [NBP2-14868] - Sample: Paraffin-embedded colon carcinoma.
Immunocytochemistry/ Immunofluorescence: Alpha Dystroglycan Antibody [NBP2-14868] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Fe, Rb, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.66 mg/ml

Order Details

Alpha Dystroglycan Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Dystroglycan. The exact sequence is proprietary.
Predicted Species
Mouse (92%), Rat (91%), Bovine (93%), Feline (94%), Canine (92%), Rhesus Macaque (97%), Rabbit (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAG1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.66 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Alpha Dystroglycan Antibody

  • A3a
  • AGRNR
  • DAG156DAG
  • dystroglycan 1 (dystrophin-associated glycoprotein 1)
  • dystroglycan
  • Dystrophin-associated glycoprotein 1

Background

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DAG1
Uniprot