alpha-Defensin 1 Antibody

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Western Blot: alpha-Defensin 1 Antibody [NBP2-27562] - Analysis of Human Spleen lysate (35ug protein in RIPA buffer) using NBP2-27562 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562] - (3.8 ug/ml) Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

alpha-Defensin 1 Antibody Summary

Immunogen
Peptide with sequence C-DESLAPKHPGSRKN corresponding to internal region according to NP_004075.1, NP_005208.1.
Specificity
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DEFA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa band according to NP_004075.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Thymus shows staining of selected cells.
Publications
Read Publication using NBP2-27562.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Defensin 1 Antibody

  • alpha 2
  • alphaDefensin 1
  • alpha-Defensin 1
  • DEF1
  • DEF1DEFA1B
  • DEFA1
  • DEFA2
  • defensin, alpha 1, myeloid-related sequence
  • defensin, alpha 1HP-1
  • HNP-1
  • HNP-1HP1
  • HP1
  • MRS
  • MRSMGC138393
  • myeloid-related sequence
  • neutrophil defensin 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Defensin 1 Antibody (NBP2-27562)(1)


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Blogs on alpha-Defensin 1.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

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Bioinformatics

Gene Symbol DEFA1
Uniprot