alpha-Aminoadipate Aminotransferase Antibody

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Aminoadipate Aminotransferase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7927) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Aminoadipate Aminotransferase at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-Aminoadipate Aminotransferase Antibody Summary

• Immunogen: E. coli-derived recombinant human alpha -Aminoadipate Aminotransferase
  Met1-Leu425
  Accession # Q8N5Z0
• Specificity: Detects human alpha -Aminoadipate Aminotransferase in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AADAT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Aminoadipate Aminotransferase Antibody

• AADAT
• alphaAminoadipate Aminotransferase
• alpha-Aminoadipate Aminotransferase
• aminoadipate aminotransferase
• EC 2.6.1.39,2-aminoadipate aminotransferase
• EC 2.6.1.7,2-aminoadipate transaminase
• KAT/AadAT
• KAT2
• KAT2mitochondrial
• Kynurenine aminotransferase II
• Kynurenine--oxoglutarate aminotransferase II
• Kynurenine--oxoglutarate transaminase II
• L kynurenine/alpha aminoadipate aminotransferase

Background

Alpha-Aminoadipate Aminotransferase (AADAT, KAT2 and Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial) is a 47 kDa (predicted) mitochondrial member of the class-I pyridoxal-phosphate-dependent aminotransferase family of proteins.  One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. Human ADDAT is predominantly expressed in the liver, it is also found in heart, brain, kidney, pancreas, prostate, testis and ovary. Over aa 1-425, human AADAT shares 73% aa sequence identity with mouse ADDAT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AADAT
• Uniprot:
  • Q8N5Z0()