Alpha Actinin 2 Antibody (EA-53)

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (EA-53) [NBP1-22630] - Formalin fixed paraffin embedded human skeletal muscle stained with alpha-Actinin, Sarcomeric antibody (NBP1-22630).

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (EA-53) [NBP1-22630] - Human skeletal muscle

• Reactivity: Hu
• Applications: IHC
• Clone: EA-53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Alpha Actinin 2 Antibody (EA-53) Summary

• Immunogen: Purified rabbit skeletal alpha-actinin.
• Localization: Cytoplasmic
• Specificity: This antibody is specific for alpha-skeletal muscle and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements. This antibody shows a wide range of cross reactivity with various mammalian species.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTN2
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20-1:30
  • Immunohistochemistry-Paraffin 1:20-1:30
• Application Notes: IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 60 min at RT.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Ascites

Alternate Names for Alpha Actinin 2 Antibody (EA-53)

• Actinin alpha 2
• actinin, alpha 2
• Actinin-a2
• ACTN2
• alpha Actinin 2
• alpha-Actinin 2
• Alpha-actinin skeletal muscle isoform 2
• Alpha-Actinin Skeletal Muscle
• Alpha-Actinin-2
• CMD1AA
• F-actin cross-linking protein

Background

Alpha actinins are actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN2
• Entrez:
  • 88(Human)