Alpha Actinin 2 Antibody (ACTN2/7041R)

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Alpha Actinin 2 Antibody (ACTN2/7041R). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: ACTN2/7041R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Alpha Actinin 2 Antibody (ACTN2/7041R) Summary

• Description: Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary) (Uniprot: P35609)
• Localization: Cytoplasm, Myofibril, Sarcomere, Z line
• Source: HEK293
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Alpha Actinin 2 Antibody (ACTN2/7041R)

• Actinin alpha 2
• actinin, alpha 2
• Actinin-a2
• ACTN2
• alpha Actinin 2
• alpha-Actinin 2
• Alpha-actinin skeletal muscle isoform 2
• Alpha-Actinin Skeletal Muscle
• Alpha-Actinin-2
• CMD1AA
• F-actin cross-linking protein

Background

Alpha actinin 2 is an actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN2