alpha 1-Acid Glycoprotein Antibody [HRP]

Product Discontinued

alpha 1-Acid Glycoprotein Antibody [HRP] Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: alpha 1-Acid Glycoprotein [Human Plasma] (Uniprot: P02763)
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alpha-1-Acid Glycoprotein [Human Plasma].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORM1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:1500
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:500
• Application Notes: This antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Theoretical MW: 23.51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555
• Control:

  alpha 1-Acid Glycoprotein Overexpression Lysate

Reactivity Notes

Cross reactivity against alpha 1-Acid Glycoprotein from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-1-Acid Glycoprotein [Human Plasma]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for alpha 1-Acid Glycoprotein Antibody [HRP]

• AGP 1
• AGP
• AGP1
• AGP1alpha-1-acid glycoprotein 1
• AGPA
• AGP-A
• alpha 1Acid Glycoprotein
• alpha 1-Acid Glycoprotein
• OMD 1
• ORM
• ORM1
• orosomucoid 1
• Orosomucoid
• orosomucoid-1

Background

Alpha 1 Acid Glycoprotein (AGP) is an acute phase plasma protein synthesized by the liver. The protein is believed to regulate the interaction between blood cells and endothelial cells, and together with haptoglobin and C reactive protein, also regulates the extravasation of the cells during infection and inflammation. AGP is a major acute phase reactant, both in human and in rat. In human plasma, AGP (44 kDa) is found at levels of 0.5-1.4 mg/ml. Its concentration in blood plasma is elevated during acute inflammation, such as trauma, malignancies, myocardial infarction, rheumatoid arthritis, bacterial infections, after major surgery, in cases of chronic pain etc, and can be used for the diagnosis of inflammatory conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for alpha 1-Acid Glycoprotein Antibody (NB100-2024) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/20/2011

Summary

• Application: Western Blot
• Sample Tested: Human recombinant protein from E. Coli
• Species: Human

Bioinformatics

• Gene Symbol: ORM1