Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - ALPL monoclonal antibody (M01), clone 4H1 Western Blot analysis of ALPL expression in HeLa ( Cat # L013V1 ).

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Mycoplasma
• Clone: 4H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALPL (AAH21289.3, 29 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSR
• Specificity: ALPL - alkaline phosphatase, liver/bone/kidney
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) - Azide and BSA Free

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase, Tissue Non-Specific Antibody (H00000249-M01)(1)

Publication using H00000249-M01

• D'Imperio L, Valera A, Naughton J et al. An extended core nanocoax pillar architecture for enhanced molecular detection. Biosens Bioelectron. 2019-03-22 [PMID: 30959392]

Bioinformatics

• Gene Symbol: ALPL
• Entrez:
  • 249(Human)
• OMIM:
  • 146300 171760 241500 241510(Human)
• Uniprot:
  • AAH21289(Human)