Alkaline Phosphatase/ALPP Antibody (SP15)

Product Discontinued

Alkaline Phosphatase/ALPP Antibody (SP15) Summary

• Immunogen: Recombinant protein encoding human placental alkaline phosphatase.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALPP
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS (pH 7.5) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Notes

For a positive control use Colon adenocarcinoma and brain.

Alternate Names for Alkaline Phosphatase/ALPP Antibody (SP15)

• Alkaline phosphatase Regan isozyme
• Alkaline Phosphatase
• alkaline phosphatase, placental (Regan isozyme)
• alkaline phosphatase, placental type
• alkaline phosphatase, placental
• alkaline phosphomonoesterase
• ALP
• ALPP
• EC 3.1.3.1
• FLJ61142
• glycerophosphatase
• PALP
• Placental alkaline phosphatase 1
• PLAP
• PLAP-1
• Regan isozyme
• SEAP

Background

Phospholipase A2 activator protein (PLAP), activates PLA2 (phospholipase A2 enzyme) and is an important mediator of eicosanoid generation. Also known as a pro-inflammatory agent, PLAP has been found in inflamed tissues and synovial fluid from patients with rheumatoid arthritis. As such, it is believed to play an important role in the regulation of inflammatory diseases. The formation of PLAP can be stimulated by IL-1 beta and TNF-alpha (1-2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Alkaline Phosphatase/ALPP Antibody (NBP1-49795). (Showing 1 - 1 of 1 FAQ).

1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
   • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Bioinformatics

• Gene Symbol: ALPP
• Uniprot:
  • P05187()