Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 388816
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Alkaline Phosphatase/ALPL
  Leu18-Ser502
  Accession # P05186
• Specificity: Detects human Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ALPL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated]

• Akp2
• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• ALPL
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALP
• TNSALPMGC167935

Background

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase/ALPL Antibody (MAB2909)(5)

Publications using MAB2909

• K Phadwal, QY Tang, I Luijten, JF Zhao, B Corcoran, RK Semple, IG Ganley, VE MacRae p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell Calcification International Journal of Molecular Sciences, 2023-01-13;24(2):. 2023-01-13 [PMID: 36675156] (Western Blot, Mouse)
• Scott Dillon, Karla Suchacki, Shun‐Neng Hsu, Louise A Stephen, Rongling Wang, William P Cawthorn et al. Ablation of Enpp6 Results in Transient Bone Hypomineralization JBMR Plus 2021-02-01 [PMID: 33615108]
• Hui Jin, Cynthia St. St. Hilaire, Yuting Huang, Dan Yang, Natalia I. Dmitrieva, Alejandra Negro et al. Increased activity of TNAP compensates for reduced adenosine production and promotes ectopic calcification in the genetic disease ACDC Science Signaling 2016-12-15 [PMID: 27965423]
• Lomashvili K, Narisawa S, Millan J, O'Neill W Vascular calcification is dependent on plasma levels of pyrophosphate. Kidney Int, 2014-04-09;85(6):1351-6. 2014-04-09 [PMID: 24717293] (Western Blot, Mouse)
• Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue. FASEB J, 2013-04-16;27(8):2977-87. 2013-04-16 [PMID: 23592762] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ALPL
• Uniprot:
  • P05186()