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ALK-1 Antibody [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ALK-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALK-1
Asp23-Pro119
Accession # Q61288
Specificity
Detects mouse ALK-1 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human ALK‑1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACVRL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse ALK-1 Antibody (Catalog # AF770)
ELISA Detection: Mouse ALK-1 Biotinylated Antibody (Catalog # BAF770)
Standard: Recombinant Mouse ALK-1 Fc Chimera (Catalog # 770-MA)
Reviewed Applications
Read 1 Review rated 5
using
AF770 in the following applications:

Publications
Read Publications using
AF770 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALK-1 Antibody [Unconjugated]

  • activin A receptor type II-like 1
  • activin A receptor, type II-like kinase 1
  • Activin receptor-like kinase 1
  • ACVRL1
  • ACVRLK1ORW2
  • ALK1
  • ALK-1
  • ALK1TGF-B superfamily receptor type I
  • EC 2.7.11
  • HHT
  • HHT2EC 2.7.11.30
  • serine/threonine-protein kinase receptor R3
  • SKR3
  • TSR-I

Background

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALK-1 Antibody (AF770)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Neutralization.


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IHC
(3)
IHC-Fr
(2)
Neutralization
(1)
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Filter By Species
Human
(1)
Mouse
(5)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 10.
Publications using AF770 Applications Species
M Morita, A Yoneda, N Tokunoh, T Masaki, K Shirakura, M Kinoshita, R Hashimoto, N Shigesada, J Takahashi, M Tachibana, S Tanaka, M Obana, N Hino, M Ikawa, K Tsujikawa, C Ono, Y Matsuura, H Kidoya, N Takakura, Y Kubota, T Doi, K Takayama, Y Yoshioka, Y Fujio, Y Okada Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models Proceedings of the National Academy of Sciences of the United States of America, 2023-01-12;120(3):e2213317120. 2023-01-12 [PMID: 36634143] (IHC, Mouse) IHC Mouse
C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020-02-10;0(0):. 2020-02-10 [PMID: 32042114] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
Wan Zhu, Daniel Saw, Miriam Weiss, Zhengda Sun, Meng Wei, Sonali Shaligram et al. Induction of Brain Arteriovenous Malformation Through CRISPR/Cas9-Mediated Somatic Alk1 Gene Mutations in Adult Mice Translational Stroke Research 10/1/2019 [PMID: 30511203]
Naoufal Akla, Claire Viallard, Natalija Popovic, Cindy Lora Gil, Przemyslaw Sapieha, Bruno Larrivée BMP9 (Bone Morphogenetic Protein-9)/Alk1 (Activin-Like Kinase Receptor Type I) Signaling Prevents Hyperglycemia-Induced Vascular Permeability Arteriosclerosis, Thrombosis, and Vascular Biology 8/1/2018 [PMID: 29880487]
PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia Nat Commun, 2016-11-29;7(0):13650. 2016-11-29 [PMID: 27897192] (IHC, Mouse) IHC Mouse
K Ntumba, N Akla, SP Oh, A Eichmann, B Larrivée BMP9/ALK1 inhibits neovascularization in mouse models of age-related macular degeneration Oncotarget, 2016-08-30;7(35):55957-55969. 2016-08-30 [PMID: 27517154] (IHC-Fr, Mouse) IHC-Fr Mouse
Qingwei Zhu, Yong Hwan Kim, Douglas Wang, S. Paul Oh, Kunxin Luo SnoN facilitates ALK1–Smad1/5 signaling during embryonic angiogenesis Journal of Cell Biology 9/16/2013 [PMID: 24019535]
Welser-Alves J, Boroujerdi A, Tigges U, Wrabetz L, Feltri M, Milner R Endothelial beta4 integrin is predominantly expressed in arterioles, where it promotes vascular remodeling in the hypoxic brain. Arterioscler Thromb Vasc Biol, 2013-03-07;33(5):943-53. 2013-03-07 [PMID: 23471230] (IHC-Fr, Mouse) IHC-Fr Mouse
Bruno Larrivée, Claudia Prahst, Emma Gordon, Raquel del Toro, Thomas Mathivet, Antonio Duarte et al. ALK1 Signaling Inhibits Angiogenesis by Cooperating with the Notch Pathway Developmental Cell 3/13/2012 [PMID: 22421041]
Mitchell D, Pobre EG, Mulivor AW, Grinberg AV, Castonguay R, Monnell TE, Solban N, Ucran JA, Pearsall RS, Underwood KW, Seehra J, Kumar R ALK1-Fc inhibits multiple mediators of angiogenesis and suppresses tumor growth. Mol. Cancer Ther., 2010-02-02;9(2):379-88. 2010-02-02 [PMID: 20124460] (Neutralization, Human) Neutralization Human

Review for ALK-1 Antibody (AF770) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF770:
Filter by Applications
IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Mouse 01/09/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22421041
SpeciesMouse

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Verified Customer
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Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ACVRL1
Uniprot