ALG3 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

ALG3 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ALG3.
  
  

  Source: E. coli

  Amino Acid Sequence: IDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIR

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: ALG3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-91658.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for ALG3 Recombinant Protein Antigen

• alpha-1,3-mannosyltransferase)
• asparagine-linked glycosylation 3 homolog (S. cerevisiae
• asparagine-linked glycosylation 3 homolog (yeast
• asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S.cerevisiae)
• Asparagine-linked glycosylation protein 3 homolog
• carbohydrate deficient glycoprotein syndrome type IV
• CDGS4D16Ertd36e
• Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
• dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
• dol-P-Man dependent alpha(1-3)-mannosyltransferase
• Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
• EC 2.4.1.130
• NOT
• Not56
• NOT56LCDG1D
• Not56-like protein

Background

The ALG3 gene encodes a member of the ALG3 family. The encoded protein catalyses the addition of the first dol-P-Man derived mannose in an alpha 1,3 linkage to Man5GlcNAc2-PP-Dol. Defects in this gene have been associated with congenital disorder of glycosyla

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ALG3