Aldolase B Antibody (OTI3C6) - Azide and BSA Free

Western Blot: Aldolase B Antibody (OTI3C6) - Azide and BSA Free [NBP2-70162] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aldolase B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aldolase B.

Immunohistochemistry: Aldolase B Antibody (OTI3C6) - Azide and BSA Free [NBP2-70162] - Staining of paraffin-embedded Human Kidney tissue using anti-Aldolase B mouse monoclonal antibody.

Flow Cytometry: Aldolase B Antibody (OTI3C6) - Azide and BSA Free [NBP2-70162] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Aldolase B antibody, and then analyzed by flow cytometry.

Immunohistochemistry: Aldolase B Antibody (OTI3C6) - Azide and BSA Free [NBP2-70162] - Staining of paraffin-embedded Human liver tissue using anti-Aldolase B mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Aldolase B Antibody (OTI3C6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ALDOB(NP_000026) produced in HEK293 cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDOB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 39.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Aldolase B Antibody (OTI3C6) - Azide and BSA Free

• ALDO2
• aldolase B, fructose-bisphosphate
• EC 4.1.2.13
• fructose-bisphosphatase
• fructose-bisphosphate aldolase B

Background

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13 ) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDOB