Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10)

Images

 
Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) [NBP1-74042] - The extracts of HepG2 (33ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 (1:1000). Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) [NBP1-74042] - Analysis in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody ...read more
Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) [NBP1-74042] - Lane 2. HepG2 cell lysate, Lane 3. Raji cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
6D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) Summary

Immunogen
Recombinant human AKR1C1 (1-323aa) purified from E. coli
Specificity
Anti-human AKR1C1 mAb, clone 6D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AKR1C1 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AKR1C1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10)

  • 20 alpha-hydroxysteroid dehydrogenase
  • 20-ALPHA-HSD
  • 20-alpha-hydroxysteroid dehydrogenase
  • 2-ALPHA-HSD
  • AKR1C1
  • Aldo-keto Reductase 1C1
  • aldo-keto reductase family 1 member C1
  • aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase)
  • AldoketoReductase 1C1
  • C9
  • Chlordecone reductase homolog HAKRC
  • DD1/DD2
  • DD1MGC8954
  • DDH1
  • DDHH-37
  • dihydrodiol dehydrogenase 1
  • Dihydrodiol dehydrogenase 1/2
  • dihydrodiol dehydrogenase isoform DD1
  • EC 1.1.1
  • EC 1.1.1.-
  • EC 1.1.1.112
  • EC 1.1.1.149,2-ALPHA-HSD
  • EC 1.3.1.20
  • HAKRC
  • HAKRCDDH1aldo-keto reductase C
  • HBAB
  • hepatic dihydrodiol dehydrogenase
  • High-affinity hepatic bile acid-binding protein
  • Indanol dehydrogenase
  • MBAB
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
  • type II 3-alpha-hydroxysteroid dehydrogenase

Background

The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AKR1C1
Entrez
Uniprot