Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-44998] - Analysis of AKR1B10 expression in RKO cell lysate (Lns 1 & 3) and HCT-8 cell lysate (Lns 2 & 4). Image courtesy of the laboratory of Dr. Deliang Cao ...read more
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NBP1-44998] - AKR1B10 expression in 1) aldose reductase (AR) protein, 2) AKR1B10 protein, 3) 293T cells expressing AR and 4) HCT-8 cells expressing AKR1B10. Image ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1 mM Tris-HCl, 100 mM sodium chloride and 50% glycerol.
Immunogen affinity purified
Western Blot 1:500-1:1000
Immunoprecipitation 5 ug
This AKR1B10 antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen sections, Immunohistochemistry paraffin embedded sections, Immunoprecipitation and Western Blot analysis where a band can be seen at ~36 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-44998 in the following applications:
Reacts with human. Does not cross react with mouse, rat or rabbit.
Alternate Names for Aldo-keto Reductase 1B10/AKR1B10 Antibody
aldo-keto reductase family 1 member B10
aldo-keto reductase family 1, member B10 (aldose reductase)
aldo-keto reductase family 1, member B11 (aldose reductase-like)
aldose reductase-like 1
aldose reductase-like peptide
Aldose reductase-related protein
Small intestine reductase
AKR1B10 (aldo-keto reductase 1B10) is a monomeric enzyme that reduces aliphatic and aromatic aldehydes as well as certain hexoses thereby playing a critical role in detoxification of dietary/cellular alpha or beta-unsaturated carbonyls and lipid peroxides (LPOs) ultimately leading to host cells protection against carbonyl lesions. AKR1B10 is active against polycyclic aromatic hydrocarbon (PAHs) which are potent procarcinogens and against all-trans-retinal, the signaling molecule retinoic acid's precursor which regulates cellular proliferation and differentiation. AKR1B10 is a cytosolic protein, but gets secreted through lysosome-mediated mechanisms and in normal tissues, it is expressed in colon, small intestine, adrenal gland etc., whereas its expression gets upregulated in lung bronchial epithelium of cigarette smokers further augmenting the risk of PAHs mediated initiation of carcinogenesis. Moreover, AKR1B10 is overexpressed in certain cancers such as liver, breast and lung cancer, and because of its reducing potential for C13 ketonic groups of daunorubicin and idarubicin, it may provide chemoresistance for cancer cells to these drugs. Moreover, upregulated AKR1B10 in malignant cells blocks ubiquitin-dependent degradation of ACCA (acetyl-CoA carboxylase-alpha) thereby promoting fatty acid/lipid synthesis which is essential to cancer cell growth and proliferation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.