ALDH5A1 Antibody (17F5) - BSA Free

Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Figure c: Downregulation of the ALDH5A1 protein product SSADH in TG1-miR. Western blot analysis. SSADH MW, 57 kDa; Actin MW, 42 kDa. Mean +/- SD, n = 3 independent biological samples. Figure d: Decreased ALDH5A1 mRNA levels in TG1-miR compared to TG1. Q-PCR assays. Mean +/- SD, n = 3 independent biological samples. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Immunohistochemistry: ALDH5A1 Antibody (17F5) [NBP2-59409] - SSADH immunoreactive cells are enriched in proliferative/non-differentiated GBM territories (PHIGH/D-) and rare in non-proliferative/differentiated (PLOW/D+) tumor territories of patients' GBM, as revealed by immunohistochemical staining. HES: hematoxylin and eosin staining. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.

Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis in HepG2 cell lysates (40ug), resolved by SDS-PAGE.

Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 17F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ALDH5A1 Antibody (17F5) - BSA Free Summary

• Description: Novus Biologicals Mouse ALDH5A1 Antibody (17F5) - BSA Free (NBP2-59409) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-ALDH5A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human ALDH5A1 (48-535aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH5A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ALDH5A1 Antibody (17F5) - BSA Free

• aldehyde dehydrogenase 5 family, member A1
• EC 1.2.1
• EC 1.2.1.24
• NAD(+)-dependent succinic semialdehyde dehydrogenase
• SSADHmitochondrial

Background

ALDH5A1 belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH5A1 Antibody (NBP2-59409)(1)

Publication using NBP2-59409

• El-Habr E A, Dubois L G et al. A driver role for GABA metabolism in controlling stem and proliferative cell state through GHB production in glioma. Acta Neuropathol 2017-01-04 [PMID: 28032215] (WB, IF/IHC, Human)

Bioinformatics

• Gene Symbol: ALDH5A1