ALDH1L1 Antibody

Product Discontinued

ALDH1L1 Antibody Summary

• Immunogen: Recombinant construct of amino acids 1 - 400 of human ALDH1L1 protein expressed in and purified from E. coli.
• Localization: Cytoplasm.
• Marker: Astrocyte Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH1L1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Western Blot 1:5000-1:10000
• Application Notes: This ALDH1L1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~100 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.07% Sodium Azide
• Purity: Unpurified

Alternate Names for ALDH1L1 Antibody

• 10-formyltetrahydrofolate dehydrogenase
• aldehyde dehydrogenase 1 family, member L1
• aldehyde dehydrogenase family 1 member L1
• Cytosolic 10-formyltetrahydrofolate dehydrogenase
• DKFZp781N0997
• EC 1.5.1.6,10-FTHFDH
• FDH
• formyltetrahydrofolate dehydrogenase
• FTHFD10-fTHF

Background

ALDH1L1 (aldehyde dehydrogenase family 1, member 1) is a part of the Aldehyde dehydrogenases (ALDH) superfamily, which is a group of enzymes that catalyze oxidation (dehydrogenation) of aldehydes. To date, nineteen ALDH genes have been identified within the human genome. They belong to 15 families in ALDH superfamily. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 expression is tissue-specific and is highly expressed in the liver, representing up to 1% of the total pool of soluble cell proteins in the mammalian liver. In an earlier study, Cahoy et al (J Neurosci.28:264-78 (2008)) applied FACS (Fluorescent-Activated Cell Sorting) to isolate astrocytes from EGFP (Enhanced Green Fluorescent Protein) transgenic-mouse, then created a transcriptome database of the expression levels of 20,000 genes by gene profiling of neurons, astrocytes and oligodedrocytes using Affymetrix GeneChip Arrays. They identified ALDH1L1 as a highly and specifically expressed gene in astrocytes. ALDH1L1 is more widely expressed throughout the brain, while astrocyte marker GFAP shows more predominant expression in white matter. In addition, loss of function or expression of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression, suggesting its role as a biomarker and a target in cancer therapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH1L1 Antibody (NBP2-25143)(2)

Publications using NBP2-25143

• Kwak H, Koh W, Kim S et al. Astrocytes Control Sensory Acuity via Tonic Inhibition in the Thalamus Neuron 2020-08-30 [PMID: 32905785]
• Koh W, Park YM, Lee SE, Lee CJ. AAV-Mediated Astrocyte-Specific Gene Expression under Human ALDH1L1 Promoter in Mouse Thalamus. Exp Neurobiol 2017-01-01 [PMID: 29302202] (Mouse)

Bioinformatics

• Gene Symbol: ALDH1L1
• OMIM:
  • 600249(Human)