ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

Western Blot: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human ALDH1A3 (NP_000648) gene (the fusion of amino acids 1-100 and 413-512) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-P

Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Flow Cytometry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Analysis of Jurkat cells, using ALDH1A3 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI4E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 413-512 of human ALDH1A3 (NP_000648) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-2000
• Theoretical MW: 55.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

• aldehyde dehydrogenase 1 family, member A3
• Aldehyde Dehydrogenase 1-A3
• Aldehyde dehydrogenase 6
• aldehyde dehydrogenase family 1 member A3
• ALDH1A3
• ALDH1A6
• ALDH6
• ALDH6acetaldehyde dehydrogenase 6
• EC 1.2.1
• EC 1.2.1.5
• MCOP8
• RALDH3
• RALDH-3
• Retinaldehyde dehydrogenase 3

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A3