alcohol dehydrogenase 5 Antibody

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Western Blot: alcohol dehydrogenase 5 Antibody [NB100-57101] - (0.5ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

alcohol dehydrogenase 5 Antibody Summary

Immunogen
Peptide with sequence C-KKIKVDEFVTHN corresponding to internal region according to NP_000662.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADH5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 38 kDa band observed in human testis lysates (calculated MW of 39.7 kDa band according to NP_000662.3). An anonymous customer has reported positive results on rat penis tissue and mouse penile tissue. IHC: An anonymous customer has reported positive results in on rat penile tissue.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-57101.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alcohol dehydrogenase 5 Antibody

  • ADH-3
  • ADHXEC 1.1.1.1
  • alcohol dehydrogenase (class III), chi polypeptide
  • alcohol dehydrogenase 5 (class III), chi polypeptide
  • Alcohol dehydrogenase 5
  • Alcohol dehydrogenase class chi chain
  • alcohol dehydrogenase class-3
  • Alcohol dehydrogenase class-III
  • EC 1.1.1
  • EC 1.1.1.-
  • EC 1.1.1.284
  • FALDH
  • FDHGSNOR
  • formaldehyde dehydrogenase
  • Glutathione-dependent formaldehyde dehydrogenase
  • GSH-FDH
  • S-(hydroxymethyl)glutathione dehydrogenase

Background

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alcohol dehydrogenase 5 Antibody (NB100-57101)(1)

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Bioinformatics

Gene Symbol ADH5
Entrez
Uniprot