Albumin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Mouse Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with at 20mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Human Liver.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Mouse Liver.Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody at 10 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Rat Liver.Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Validation in Rat Liver. Loading: 10 ug of lysates per lane.Antibodies: Albumin, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Validation in Human Liver.Loading: 10 ug of lysates per lane.Antibodies: Albumin, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Loading: 10 µg of lysates per lane.Antibodies: Albumin, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Albumin Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin. The immunogen is located within amino acids 340 - 390 of Albumin. Amino Acid Squence: LYEYARRHPDTSV
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: ALB
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  Albumin Protein (NBP1-77027PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Donkey (85%), Horse (85%), Pig (85%), Rabbit (85%), Chicken (80%).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Albumin Antibody - BSA Free

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (NBP1-77027)(3)

Publications using NBP1-77027

• Kermode-Scott B., , et Al. Terry-Nan Tannenbaum BMJ 2016-08-30 [PMID: 27102115]
• da Silva CG, Studer P, Skroch M et al. A20 promotes liver regeneration by decreasing SOCS3 expression to enhance IL-6/STAT3 proliferative signals. Hepatology 2013-05-01 [PMID: 23238769] (IF/IHC, ICC/IF, Mouse)
• Rothschild MA, Oratz M, Schreiber SS. Serum Albumin. Hepatology. [PMID: 4568596]

Bioinformatics

• Gene Symbol: ALB
• Entrez:
  • 213(Human)
  • 11657(Mouse)
  • 24186(Rat)
• Uniprot:
  • P02768(Human)