Albumin Antibody (1G12-1B3) - Azide and BSA Free

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01] - Analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody (M01), clone 1G12-1B3.Lane 1: ALB transfected lysate(69.4 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Albumin Antibody (1G12-1B3) [H00000213-M01] - Analysis of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. Antibody concentration 3 ug/ml.

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01] - ALB expression in HepG2 ( Cat # L019V1 ).

Immunoprecipitation: Albumin Antibody (1G12-1B3) [H00000213-M01] - Analysis of ALB transfected lysate using anti-ALB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALB MaxPab rabbit polyclonal antibody.

ELISA: Albumin Antibody (1G12-1B3) [H00000213-M01] - Detection limit for recombinant GST tagged ALB is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 1G12-1B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Albumin Antibody (1G12-1B3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALB (AAH34023, 19 a.a. ~ 609 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEGGKKLVAASQAALGL
• Specificity: ALB - albumin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Albumin Antibody (1G12-1B3) - Azide and BSA Free

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (H00000213-M01)(3)

Publications using H00000213-M01

• Simeonov KP, Uppal H. Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs. PLoS One. 2014-06-25 [PMID: 24963715]
• Nagai S, Saito Y, Endo Y et al. Hypoalbuminemia in early onset dentatorubral-pallidoluysian atrophy due to leakage of albumin in multiple organs. J Neurol. 2012-12-23 [PMID: 23263592]
• Jackson MI, Cao J, Zeng H et al. S-Adenosylmethionine Dependent Protein Methylation is Required for Expression of Selenoprotein P and Gluconeogenic Enzymes in Human Hepatocytes. J Biol Chem. 2012-08-29 [PMID: 22932905]

Bioinformatics

• Gene Symbol: ALB
• Entrez:
  • 213(Human)
• OMIM:
  • 103600(Human)
• Uniprot:
  • AAH34023(Human)